Abstract: Using digital waveform data recorded by regional seismic networks in Yunnan between 2011 and 2015, attenuation characteristics and site response respectively are calculated. The frequency dependent Q(f) is obtained by the iterative grid-search technique, as described by Atkinson and Mereu, based on trilinear geometrical spreading model. Our results demonstrate that for Yunnan the associated model for regional quality factor for frequencies can be expressed as Q(f)=193.8f0.528. Additionally, we ascertain the source spectra determined by the model of Brune and the site responses of seismic stations derived by Moya’s method using genetic algorithms. The site response results of 46 stations demonstrate that the seismic signal amplification of Station bedrock is not the same in different frequency bands. The site response in Yunnan region shows obvious amplification with low frequency and attenuation with high frequency. Site amplifications are apparent in excellent frequency band (1~4 Hz), mostly between 1 and 10 times, and in the high frequency (10 Hz or more) site attenuation is mostly between 0.05 and 1 times.